novus biologicals nbp2 30169  (Novus Biologicals)

Journal: International Journal of Molecular Sciences

Article Title: Preclinical Efficacy of a PARP-1 Targeted Auger-Emitting Radionuclide in Prostate Cancer

doi: 10.3390/ijms24043083

Figure Lengend Snippet: Increase in PARP-1 protein expression with Gleason grade in prostate cancer tissues. ( A ), immunohistochemistry (IHC) staining of PARP-1 in normal human prostate, prostate hyperplasia, and prostate cancer cases graded based on Gleason score in a tissue microarray (TMA). Representative photomicrographs at 4× (top panel) and 40× (lower panel) magnification are shown. ( B ), IHC scoring of PARP-1 expression in the TMA cores. Scatter plots showing the distribution of PARP IHC scores. IHC score is calculated as the sum of the PARP-1 positive proportion (0 = no positive tumor cells; 1 = <1%; 2 = 1–10%; 3 =11–33%; 4 = 34–66%; 5 = 67–100%) and the staining intensity (0, no staining; 1, weak; 2, moderate; 3, strong; 4, very strong) for a possible total score of nine. * p < 0.05, ** p < 0.01 and **** p < 0.0001.

Article Snippet: The prostate cancer tissue microarray (TMA) was purchased from US Biomax, Inc. (Rockville, MD, USA).

Techniques: Expressing, Immunohistochemistry, Microarray, Staining

Journal: The Journal of Biological Chemistry

Article Title: Peroxiredoxin IV plays a critical role in cancer cell growth and radioresistance through the activation of the Akt/GSK3 signaling pathways

doi: 10.1016/j.jbc.2022.102123

Figure Lengend Snippet: Examination of Prx4 protein levels in patient specimens of prostate cancer by tissue microarray or established prostate cancer cell lines by Western blotting. A , tissue microarray slides and representative images of anti-Prx4 staining of prostate normal and adenocarcinoma. Positive anti-Prx4 staining shows in dark brown and counter staining for nuclear shows light blue . The scale bars represent 5 mm (whole slide), 500 μm (individual tumor), and 100 μm (zoomed in). B and C , anti-Prx4 staining intensity were quantitatively scored by board-certified pathologist and data were compared between prostate normal (n = 50) and adenocarcinoma (n = 66) ( B ), as well as between prostate normal and cancer with different levels of Gleason grading ( C ). D , examination of Prx4 levels in prostate derived nontumorigenic or tumor cell lines by Western blotting. The bar graph on the right shows quantitative data from three independent blots. ∗ p < 0.05 by two-way ANOVA. Prx, peroxiredoxin.

Article Snippet: Human prostate normal and prostate cancer tissue microarray slides were commercially obtained, including BNS19011 and PR803d (US Biomax).

Techniques: Microarray, Western Blot, Staining, Derivative Assay